glutamate siah1 sirna Search Results


glutamate siah1 sirna  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology glutamate siah1 sirna
Figure 2 The nuclear translocation of GAPDH upon GluR2 AMPA receptor activation depends on Siah-1. A. Western blots verification of Siah 1 siRNA knockdown of the Siah-1 expression in HEK 293 T cells. B. Western blots analysis of GAPDH expression in the nucleus with/without glutamate treatment in the presence/absence of the <t>Siah1</t> siRNA (Santa Cruz) in HEK-293 T cells expressing GluR1/2 subunits. LaminB1 was used as the nuclear marker. C & D, Quantification of nuclear expression of GAPDH with/without glutamate treatment in the presence/absence of the Siah1 siRNA (Santa Cruz), three Siah1 siRNA (#1, #2, #3, OriGene) and scrambled negative control siRNA (scramble, OriGene). *p < 0.05; significantly different from control group (n = 3 per group). #p < 0.05; significantly different from glutamate group (n = 3 per group). ANOVA, followed by post-hoc SNK test.
Glutamate Siah1 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glutamate siah1 sirna/product/Santa Cruz Biotechnology
Average 91 stars, based on 1 article reviews
glutamate siah1 sirna - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier

Image Search Results


Figure 2 The nuclear translocation of GAPDH upon GluR2 AMPA receptor activation depends on Siah-1. A. Western blots verification of Siah 1 siRNA knockdown of the Siah-1 expression in HEK 293 T cells. B. Western blots analysis of GAPDH expression in the nucleus with/without glutamate treatment in the presence/absence of the Siah1 siRNA (Santa Cruz) in HEK-293 T cells expressing GluR1/2 subunits. LaminB1 was used as the nuclear marker. C & D, Quantification of nuclear expression of GAPDH with/without glutamate treatment in the presence/absence of the Siah1 siRNA (Santa Cruz), three Siah1 siRNA (#1, #2, #3, OriGene) and scrambled negative control siRNA (scramble, OriGene). *p < 0.05; significantly different from control group (n = 3 per group). #p < 0.05; significantly different from glutamate group (n = 3 per group). ANOVA, followed by post-hoc SNK test.

Journal: Molecular brain

Article Title: Disruption of the nuclear p53-GAPDH complex protects against ischemia-induced neuronal damage.

doi: 10.1186/1756-6606-7-20

Figure Lengend Snippet: Figure 2 The nuclear translocation of GAPDH upon GluR2 AMPA receptor activation depends on Siah-1. A. Western blots verification of Siah 1 siRNA knockdown of the Siah-1 expression in HEK 293 T cells. B. Western blots analysis of GAPDH expression in the nucleus with/without glutamate treatment in the presence/absence of the Siah1 siRNA (Santa Cruz) in HEK-293 T cells expressing GluR1/2 subunits. LaminB1 was used as the nuclear marker. C & D, Quantification of nuclear expression of GAPDH with/without glutamate treatment in the presence/absence of the Siah1 siRNA (Santa Cruz), three Siah1 siRNA (#1, #2, #3, OriGene) and scrambled negative control siRNA (scramble, OriGene). *p < 0.05; significantly different from control group (n = 3 per group). #p < 0.05; significantly different from glutamate group (n = 3 per group). ANOVA, followed by post-hoc SNK test.

Article Snippet: As shown in Figure 3D, the ability of GAPDH antibody to co-immunoprecipitate with p53 was significantly blocked by pre-incubation with the I221-E250 peptide, indicating the I221-E250 region is essential in maintaining p53-GAPDH coupling. p53-GAPDH interaction plays an important role in AMPA receptor-mediated cell death We hypothesized that translocation of GAPDH to the nucleus following glutamate stimulation, may subsequently A B GAPDH LaminB1 * # 0 100 200 300 400 500 600 700 R el at iv e In te n si ty o f n u cl ea r G A P D H ( P er ce n t C o n tr o l) Glutamate Siah1 siRNA (SCBT) - - + + - + 0 100 200 300 400 500 600 700 R el ev an t in te n si ty o f G A P D H (P er ce n t C o n tr o l) + + + + + - - scramble #1 #2 #3 - Glutamate siah1 siRNA (OriGene) * * # # # C D -Siah1 siRNA + Siah1 Tubulin Figure 2 The nuclear translocation of GAPDH upon GluR2 AMPA receptor activation depends on Siah-1.

Techniques: Translocation Assay, Activation Assay, Western Blot, Knockdown, Expressing, Marker, Negative Control, Control